Sentence examples for by immunofluorescence microscopy using from inspiring English sources

Since the two TSR proteins reported to be O-fucosylated in sporozoites are CSP and TRAP7, we investigated these two proteins by immunofluorescence microscopy using antibodies directed to these proteins (Fig. 4a).

We report here that SV40-specific proteins can be visualised on the cell surface of Ad2+ND2 infected HeLa cells by immunofluorescence microscopy using a rabbit anti-T serum, prepared against purified SDS-denatured T-antigen.

To examine mitotic cells after cdr2 knockdown, we imaged mitotic spindles in these cells by immunofluorescence microscopy, using a α-tubulin antibody and DAPI to visualize mitotic cells.

The presence of virus in infected cells was confirmed by immunofluorescence microscopy using monoclonal antibodies specific for flavivirus E proteins [46] and by RT-PCR.

72 hours later puromycin was removed and cells were cloned by serial dilution and checked for PML expression by immunofluorescence microscopy using antibody against all PML isoforms.

VMR-CM-stimulated fibroblasts were then analyzed by immunofluorescence microscopy using antibodies against the lysosomal marker LAMP-1 and S100A4 (Fig. 2B).

Z-stacks of GFP-expressing invading cells were obtained by indirect immunofluorescence microscopy using a Zeiss LSM710 confocal microscope.

In order to identify any biological consequence of the FYVE-CENT R1945Q mutation, we examined the phenotype of mutant cells by performing immunofluorescence microscopy using the HCC-1954 and HCC-1395 breast cancer cells.

To see if Nullbasic similarly localizes to the cytoplasm, HeLa cells expressing Nullbasic or the wild type Tat-FLAG fusion protein were visualized by indirect immunofluorescence microscopy using anti-FLAG antibody.

Low levels of progerin were also detected by indirect immunofluorescence microscopy using a specific antibody in a restricted number of dermal fibroblasts in skin sections derived from elderly subjects (Fig. 6C).

To assess encystation in the presence of Bax, we compared ESV formation in wild type and transgenic Giardia by confocal immunofluorescence microscopy using an antibody against CWP1, a major ESV cargo protein.