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When we treated the AFiPSCs with a combination of 10 ng/ml BMP4 and 10 µM SB431542 over a period of seven days, the same morphological changes could be observed and human chorionic gonadotropin (hCG), a hormone secreted by trophoblastic cells of the placenta, was detected by immunofluorescence microscopy analysis (Figure 3C).
We further characterized the effect of CTα depletion on infection by immunofluorescence microscopy analysis of thick liver sections.
By immunofluorescence microscopy analysis using markers such as GATA4 and Oct3/4, we determined that 80 90% of the ES cell population had differentiated into extraembryonic endoderm-like cells after treatment with retinoic acid (Fig. 1B,C).
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As shown by high-resolution, immunofluorescence microscopy analysis of CD59 and stomatin localization, SMase treatment not only led to morphological changes, but also to a reorganization of membrane microdomains.
EC was involved in spinner experiments and immunofluorescence microscopy analysis.
For immunofluorescence microscopy analysis, the number of cells was greater than 10.
Cell surface Fas expression was assessed by immunofluorescence microscopy and FACS analysis.
H3K36me3 was detected by immunofluorescence microscopy and Western analysis (Additional file 2A) at advanced macronuclear anlagen (a3) with high level of polyteny.
Uptake of these vesicles, under different conditions, by the same cells from where they originated was monitored by immunofluorescence microscopy and flow cytometry analysis.
To investigate a possible role for glycosylation in exosome uptake Exos-CFSE were desialylated with V. cholerae and A. urefaciaens sialidases and exosomes uptake was monitored by immunofluorescence microscopy and flow cytometry analysis of cell fluorescence intensity.
The cell biological analysis of GFAP levels by immunofluorescence microscopy also confirmed the proteomic analysis of the mdx- 4cv brain.
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