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Retinal expression of EGFP following subretinal delivery of CMV-EGFP (CK30PEG pZEOGFP5.1) nanoparticles was examined by immunofluorescence labeling using anti-GFP antibody.
In order to determine whether the cytotoxic effects of Ure2 aggregates were exerted from inside or outside the cells, we followed the fate of the Ure2 protein by immunofluorescence labeling using an antibody that recognizes both native and fibrillar states of Ure2.
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Initially, highly differentiated primary hypothalamic neuronal cultures were exposed to AβOs (500 nM) for 3 h and AβO binding to neurons was investigated by double immunofluorescence labeling using oligomer-sensitive antibody NU4 (Lambert et al, 2007) and microtubule-associated protein 2 (MAP-2).
To confirm the interaction of these cytokines in macrophage differentiation at the site of inflammation, ST samples from five patients with RA were analysed by two-color immunofluorescence labeling using Abs against IL-10R1, M-CSFR, and TNF receptors, as well as anti-CD68 Ab. Figure 7 shows representative staining patterns in RA ST.
Sections from different experimental groups were subjected to Hematoxylin and eosin (H&E) or PAS (periodic acid Schiff) staining or subjected to immunofluorescence labelling using antibodies to PAR followed by FITC-labelled secondary antibodies.
Predictions relating to cell growth, virus infection efficiency (tracked by immunofluorescence labeling), replication kinetics, substrate consumption, and cell death in suspended batch cultures agreed well with experiments.
Its surface display was confirmed by immunofluorescence labeling of the RGS (His 6 reporter inserted, and its potential was also evaluated.
TRPV1 protein was detected by immunofluorescence labeling.
In general, the signals obtained by immunofluorescence labeling followed the patterns observed by immunohistochemical staining.
Expression of iNOS was evaluated by immunofluorescence labeling in LPS-activated BV-2 microglia).
This difference in expression was confirmed by immunofluorescence labeling on fixed cardiomyocytes (Fig. 5).
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