Sentence examples for by immunofluorescence analysis using from inspiring English sources
Caspase-3 activation was analyzed by immunofluorescence analysis using a specific anti-active caspase-3 antibody.
Calmodulin is included in Table 1 because it was shown to be localized in the CV of T. cruzi by immunofluorescence analysis using human antibodies [5] and is present in the CV of D. discoideum [22] and Paramecium multimicronucleatum [23].
Nuclear translocation of the p65 subunit was demonstrated by immunofluorescence analysis (using a confocal laser microscope) in U937 cells after 3-h treatment with either 27-OH or HNE.
This was confirmed both by a colourimetric assay, in which the activity of the enzyme was detected directly in unfixed cryosections, and by immunofluorescence analysis using an antiserum directed against β-gal.
To investigate if the additional 24 aa found in BmfCUG, predicted to contain an additional α-helix, may define differences in subcellular localization we transiently transfected HA-tagged versions of the individual isoforms into 293T HEK cells or NIH3T3 fibroblasts, followed by immunofluorescence analysis using an epitope tag-specific antibody.
Next we examined the effect on the F-actin content microscopically by performing immunofluorescence analysis using phalloidin staining of F-actin.
The surface expression level of CD317 was quantified by indirect immunofluorescence analysis using the QIFIKIT (Dako, Glostrup, Denmark) and mouse CD317 monoclonal antibody (clone 26F8, eBioscience, San Diego, CA, USA) as primary antibody according to the manufacturer's protocol.
Observation was carried out by ELISA and immunofluorescence analysis using a confocal laser microscope.
The presence of these cells was demonstrated by flow cytometry and immunofluorescence analysis using von Willebrand Factor (vWF) and CD31/PECAM-1 markers.
Positive hybridomas were selected and the supernatants of the primary clones and subsequent subclones were screened by Western blot and indirect immunofluorescence analysis using control and HGPS dermal fibroblasts treated or untreated with FTI as described previously [30].
Despite the fact that pregnancy can induce ductal morphogenesis at the late stage of pregnancy, underdeveloped mammary glands in SirT1ko/ko female mice cause a severe deficit in milk production, as shown by histological analysis as well as the immunofluorescence analysis using an anti-milk antibody.
