Sentence examples for by immunoblot analyses using from inspiring English sources
The silencing efficiency was detected by immunoblot analyses using the anti-ubiquitin antibody.
We first confirmed that the HEK293s cell system properly functioned by immunoblot analyses using the anti-rhodopsin 1D4.
The serotype O1 V. cholerae strain A1552 was shown to express detectable levels of Hcp as determined by immunoblot analyses using polyclonal anti-Hcp antiserum.
We also investigated the expression levels of the CRP, HapR and RpoN proteins in the ΔrpoS and ΔrpoN derivatives by immunoblot analyses using the same samples that were used for the detection of Hcp (Fig. 7).
OATP proteins were quantified in membrane fractions from the liver samples by immunoblot analyses using previously characterized antibodies [ 28, 29] (Additional file 1).
We treated HeLa cells with a LRPPRC-specific siRNA to suppress the expression of LRPPRC and always achieved a high efficiency of suppression as confirmed by immunostaining or immunoblot analyses using an LRPPRC-specific monoclonal antibody (1B8) [ 38].
Protein levels visualized by immunoblot analyses were quantified using the NIH National Institutes of Healthh) ImageJ software (http://rsb.info.nih.gov/ij/) and normalized against the GAPDH loading control as described in Uittenbogaard et al. (2010).
Expression levels of AQP1 were controlled with an adenovirus expression system and monitored by immunoblot analyses.
METHODS: AQP1 in human RPE in situ was determined after biotinylation of proteins on cell surfaces and streptavidin chromatography, followed by immunoblot analyses.
Protein lysates (30 μg) were separated by 7.5% SDS PAGE, immunoblotted, and analysed using chemiluminescence (Amersham Biosciences, New Jersey, USA).
