Sentence examples for by immortalization of primary from inspiring English sources

The Oli-neu cell line was derived by immortalization of primary cultures of enriched oligodendrocytes with the t-neu oncogene.

The mouse N20.1 cell line (Verity et al., 1993) was derived by immortalization of primary cultures of enriched oligodendrocytes with a temperature-sensitive form of SV40 large T antigen.

To that end, we utilized two systems that were previously established in our lab by immortalization of human primary mesenchymal cells.

Diaphragm adult fibroblasts were obtained by SV40 immortalization of primary cells from wild-type and Ppif−/− mice; mouse embryo fibroblasts (MEF) from wild-type and VDAC1/3−/− mice were obtained as described [37].

Diaphragm adult fibroblasts were obtained by SV40 immortalization of primary cells from wild-type and Ppif−/− mice [27]; apoptosis inducers were added to exponentially growing cells in the absence of serum.

Fibroblast cell lines were generated by spontaneous immortalization of primary mammary fibroblasts, as described [ 49].

The immortalized primary epithelial cell line RWPE1 was a generous gift from Dr. Watson (Dublin), EP156 cells were established by hTERT immortalization of primary epithelial prostate cells [ 23].

EBNA1 is essential for the immortalization of primary B-lymphocytes by EBV infection [5], and its inhibition by siRNA depletion or by ectopic expression of dominant negative mutants induce apoptosis in EBV-infected cells [6], [7].

Immortalization of primary MEFs was performed by infection of pBABE-retroviruses bearing SV40 large T antigen (Addgene) and selection in 2.5 μg/ml puromycin (Sigma).

The E7 protein is then persistently expressed and causes the immortalization of primary keratinocytes, leading to terminally differentiated, immortalized clones [ 9].

Overexpression of HMW FGF2s leads to immortalization of primary cells in culture and allowed proliferation of immortalized cells in low-serum to achieve high cell density [ 99- 101].