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The stained cells were examined with an inverted microscope (Nikon eclipse, TE2000-U), and images were taken with a CCD camera, followed by image analysis with SPOT™ imaging software.
Some cameras evaluate the coincidence (or lack thereof) between two rangefinder images by image analysis with a microchip system.
The pictures were used to determine the length of the animals by image analysis with the image processing program ImageJ® (Wayne Rasband, National Institutes of Health).
From scanning electron micrographs, the average fibre diameter as well as their density and distribution was estimated quantitatively and qualitatively as shown in Table 2 by image analysis with Image J. The fibre density was quantified as 'mesh size' indicating the average distance of fibres in horizontal direction.
Measurement was performed using the drop shape analysis system (DSA 100, Krüss GmbH, Germany) by video documentation of the fall down of a water drop (3 µl volume, 40 µl s−1 rate) on the bacterial-covered filter surface The contact angle θ between water/bacterial surface/air was measured by image analysis with the DSA software using the circle fitting mode.
Films were scanned, and protein bands quantified by image analysis with the Image J software.
Similar(37)
An excellent correlation was evident between the percentage positive tumour nuclei as determined by image analysis compared with determined by manual analysis (Spearman's ρ = 0.9, P < 0.001).
Quantification of the separate phase size distributions by image analysis was coupled with the individual phase oxidation response to evaluate the overall oxidation behavior and phase interaction effects.
Following this initial validation stage, the ER and PR expression levels as determined by image analysis were compared with the manual assessment of the two cohorts outlined above.
To further validate the algorithm, the prognostic power of the ER and PR expression as determined by image analysis was compared with that derived from standard pathological assessment.
A 5% cutoff threshold for the PR was associated with similar HRs to manual analysis; however, an automated 10% threshold as determined by image analysis was not associated with a significant outcome.
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