Sentence examples for by identifying primer sets from inspiring English sources
Second, we simplified the PCR step by identifying primer sets that all work at identical amplification conditions, and that are tolerant of being added to reactions by pin-replication.
Genomic deletions RD105, RD142, RD150 and RD181 were identified by PCR using primer sets as previously described [5] and the PCR products were analysed by agarose gel electrophoresis.
Table 1 indicates the CoV genera identified by using individual primer sets.
Further correspondences were identified by cross-checking primer sets against the Onion Gene Index [ 39] using the primersearch tool from the EMBOSS suite [ 40] and creating correspondences for any marker pairs amplifying the same sequence.
ZAS-1 and T. azotonutricium, both of which have a second CooS grouping within clade A. While the clade A-associated cooS genes in both of these species were identified by our primers, our primer sets did not amplify the clade C-associated cooS gene in either species, which were later identified by sequencing the genomes of these spirochetes.
We identified a total of nine cDNA bands expressed at the biggest different levels in the small intestines of W/W V mice by using primer sets, which were not tested in the previous study.
It was observed that direct sequencing data of the hypervariable segment (HVS) 1 by using primer sets that generate an amplicon of 407 bp (long-primer sets) was different from results obtained by using newly designed primer sets that produce an amplicon of 120 139 bp (mini-primer sets).
Promoter annotation was performed by first identifying the sequences amplified by each primer set (Table 2) using the Ensembl Genome Browser and then inputting those sequences into Tfsitescan (http://www.ifti.org/).
Genomic PCR was performed by using two primer sets (set 1: forward primer, 5'-ATGGCTTCTAGCTATCCTTATG-3', and reverse primer, 5'-GTAGCAACCTGTGCAATTTCTTC-3'; set 2: forward primer, 5'-CTGCTAACCATGTTCATGCC-3' and reverse rimer, 5'-ACAAGTTTAGCTCCAATGTTTTTGTA-3').
BAC pools were constructed and screened by PCR with 96 EST primer sets to identify 16 BACs that contained protein-coding loci.
