Sentence examples for by homogenizing the samples from inspiring English sources

Flurometric assay was performed by homogenizing the samples in extraction buffer (50 mM NaPO4, pH 7.0; 10 mM β-mercaptoethanol; 1 mM Na2EDTA; 0.1% Sodium Lauryl Sarcosine; 0.1% Triton×100).

Sulfate was extracted by homogenizing the samples in 1 10 (w/v) ice-cold 0.1 N HNO3.

Nitrate was extracted from the tissues by homogenizing the samples previously boiled in 4 volumes of distilled water for 15 min. The homogenate was centrifuged at 12,000 g for 20 min to obtain a clarified supernatant.

Protein extraction was facilitated by homogenizing the samples with a pestle in 4× loading buffer, (200 mMTris (adjusted to pH 6.8), 10% SDS, 50% glycerol, 400 mM DTT, 0.1% Coomassie Blue, in deionized water).

Total proteins were extracted as previously described by Martínez and co-workers [ 87] by homogenizing the samples, previously powdered in liquid nitrogen, in 4 volumes of a 125 mM pH 8.8 Tris-HCl buffer containing 1% (w/v) SDS, 10% (w/v) glycerol, 50 mM Na2S2O5.

Total RNA extraction was performed by homogenizing the fat tissue samples with TRIZOL® (TRI reagent, Invitrogen, Carlsbad, CA, USA) following the manufacturer's instructions for samples with high fat content.

Cardiac tissue extracts were obtained by homogenizing the left ventricle samples in phosphate-buffered saline (PBS; 0.14 M NaCl, 3 mM KCl, 1.4 mM KH2PO4, 14 mM K2HPO4) at a concentration of 1 mg tissue/10 μL PBS for 5 min. The homogenates were placed on ice for 10 min and then centrifuged at 12,000 rpm for 30 min. The supernatant was collected and stored at –70°C for further experiments.

After homogenizing the sample of patients by propensity score, an effect on mortality is discarded and we observed a significant need for use of norepinephrine and a nonsignificant trend for prolonged mechanical ventilation and renal failure requiring renal replacement therapy, both probably related with the greatest need for vasopressors observed.

DNA from the contemporary samples was extracted by homogenizing the wing fragment in a solution of 100 μl 5% chelex-TRIS (10 mM) and 5 μl proteinase K (0.75 units).

Contents were mixed using a spatula to homogenize the samples.

To homogenize the sample, only class I subjects were selected.