Sentence examples for by homogenization in a from inspiring English sources

Spheroplasts were disrupted by homogenization in a Dounce homogenizer in a medium containing 50 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol, 1 mM EGTA, 1 mM phenylmethyl sulfonylfluoride and 1 µg/ml each of leupeptin, pepstatin, and aprotinin.

Hamster brain homogenates were processed by homogenization in a MES buffer (25 mM MES, 150 mM NaCl, 60 mM n-octyl-glucoside and 1% Triton X-100, pH 6.5) at 4 C and protein concentration quantified by BCA (Pierce, IL).

Cells were harvested as described above and were disrupted by homogenization in a ground-glass homogenizer fitted with a ground-glass pestle, using a buffer consisting of 154 mM NaCl and 10 mM sodium-potassium phosphate (pH 7.4).

Differentiating cells were harvested after 6 days of exposure, as described above, and were disrupted by homogenization in a ground-glass homogenizer fitted with a ground-glass pestle and using a buffer consisting of 154 mM NaCl and 10 mM sodium-potassium phosphate (pH 7.4).

In this way, total lipids from a known weight of tissue was extracted and purified by homogenization in a suitable excess of chloroform/methanol/water (2 2 1.8 v/v/v).

One ml lysing buffer (Qiazol, Qiagen) and a 5 mm metal bead (Millipore) were added and immediately followed by homogenization in a TissueLyser (Qiagen), with shaking three times for 2 minutes at a frequency of 25 Hz.

Hepatic triglycerides were extracted from 50 mg of liver homogenates by homogenization in 1 ml of chloroform-methanol (2∶1 v/v) using TissueLyser (Qiagen).

RNA was isolated from each tumor by homogenization in Trizol (Invitrogen) using a Tissuemizer (Tekmar Company, Cincinnati, OH).

Extracts from tissue were prepared by homogenization in 9 g/L NaCl at 4°C using a DY89-1 tissue homogenizer.

Total RNA was prepared from fetal lung by homogenization in TRIzol reagent (Gibco/BRL, Rockville, MD), a guanidinium isothiocyanate/phenol-based solution.

Hamster brain homogenates (10%) were prepared by homogenization in 320 mM sucrose and pre-cleared by centrifugation at 3000 RPM for 10 min at 4 C. Resulting supernatant was processed for inoculation by 1∶10 dilution in 320 mM sucrose or PuraMatrix (1% w/v synthetic 16-mer RADA peptide hydrogel) to yield a 1% brain homogenate (dose equivalent 10−2) in 0.9% peptide hydrogel.