Sentence examples for by homogenization and mixed from inspiring English sources

The pellet was purified membranes which were resuspended in 2 mL gradient buffer by homogenization and mixed with 1.9 mL Percoll (Amersham Biosciences, Uppsala, Sweden containing 10% PBS and 0.19 mL 2.5 M sucrose in an Easy-Seal tube (polyallomer, 5 mL, Sorvall).

Cardiac tissue lysates were prepared by homogenization and sonication with a Brickmann Polytron homogenizer and a Fisher Sonic Dismembrator.

Plant extract was prepared by homogenization and centrifugation.

Nuclei were isolated by homogenization and digested with micrococcal nuclease (MN) for 30 min at 37°C.

At the end of experiments mice were humanely killed, their feet removed and parasite burdens determined by homogenization and counting.

Total RNA was extracted from all samples by homogenization and cell lysis using TRIzol (Invitrogen, Carlsbad, CA).

Cells were disrupted by Dounce homogenization and fractionated by differential velocity centrifugation as previously described [ 7, 9].

Antiradical activity improvement by trehalose was conditioned by homogenization pressures and specific bioactive compounds.

Perhaps by preventing homogenization and minimizing bacterial interactions over the egg surface, birds can more effectively prevent infection.

Sporozoites were released from the glands by gentle homogenization and washed three times before enumeration.

Organs were harvested at the end of the experiment, and mitochondria isolated by tissue homogenization and differential centrifugation.