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SNP discovery by highly parallel sequencing was successfully demonstrated in cattle [ 15], in which reduced-representation pooled libraries were sequenced using the Illumina/Solexa technology.
Currently a number of large scale projects are under-way with the aim of genome-wide, systematic identification of functional elements, through a combination of biochemical assays followed by highly parallel sequencing [ 4].
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Within the CRoPS system, tagged complexity-reduced fragment libraries generated by the AFLP process [ 14] was sequenced by 454 highly parallel sequencing technology, and the sequences were aligned and mined for polymorphisms.
We have generated more than 300,000 novel C. elegans EST sequences by this highly parallel sequencing method for this study.
We used the transcripts generated by the highly parallel sequencing described above, as well as the already publicly available sequences to create a microarray specific to M. p. furo.
This has been integrated with sequence reads generated by a scalable, highly parallel sequencing by synthesis (SBS) method with throughput significantly greater than capillary electrophoresis.
Margulies et al. [ 40] recently described a highly parallel sequencing by synthesis approach that demonstrated an increased throughput for sequencing of genomic DNA.
Highly parallel sequencing technologies have become important tools in the analysis of sequence polymorphisms on a genomic scale.
Subsequently, the circularized target sequences are amplified in multiplex and analyzed by using a highly parallel sequencing-by-synthesis technology.
Cölfen, H. & Antonietti, M. Mesocrystals: inorganic superstructures made by highly parallel crystallization and controlled alignment.
NGS can be characterized by highly parallel operation, higher yield, simpler operation, shorter reads, and much lower cost [ 10].
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