We previously showed that follistatin can also block myostatin activity in vivo; specifically, we showed that follistatin can ameliorate the cachexia induced by high level expression of myostatin in nude mice [21] and that transgenic mice expressing follistatin in muscle have dramatic increases in muscle mass [19].
These data show that antibodies against cholera toxin can be induced in vivo by high level expression of a non toxic mutant, or by using a mutant with residual ADP-ribosyltransferase activity.
To narrow down which HDACs are directly involved in cytarabine sensitivities in pediatric AMLs, we used equal doses (IC20s, determined by MTT assays) of the above HDACIs (MS-275, VPA, and SAHA) with diverse substrate specificities to treat THP-1 cells, characterized by high level expression of both class I and II HDACs.
Lipogenic breast tumors, defined by high level expression of FASN, carry a poor clinical prognosis [ 62], but it is not clear whether expression of FASN plays a causal role in disease or whether it reflects other underlying metabolic changes in such tumors.
Conversely, the myoblasts forming the direct flight muscles (DFMs), which are located in adjacent areas near the future wing hinge, are marked by high-level expression of the homeobox gene cut.
Applying these assays to the TCGA cohort revealed distinct clusters of outliers characterized by high-level expression of mutant transcript (Fig. 3a c).
MaviP35 inhibited yeast death triggered by high-level expression of DRONC, suggesting that it could function as a pseudo-substrate inhibitor of DRONC.
Therefore, the defects observed in 35S NTAP AtCTF7∆B plants are caused by high-level expression of AtCTF7∆B and not the presence of the NTAP tag.
Transgenic plants expressing 35S AtCTF7∆B displayed similar vegetative defects, suggesting the defects in 35S NTAP AtCTF7∆B plants are caused by high-level expression of AtCTF7∆B.
This might be due to the elevated VEGF secretion induced by high-level expression of Bcl-2 in response to hypoxic condition [ 36].
Pyruvate pools in ethanologenic E. coli are substantially reduced by high-level expression of these Z. mobilis genes, but cells adjust their metabolism to various levels along the glycolytic pathway to fulfill the carbon flux.
