Sentence examples for by heating the template from inspiring English sources

Melting curve analyses were performed by heating the template at 95°C with a 0 s hold, then cooling to 60°C with a 15 s hold, and finally increasing the temperature to 95°C with a 0.1°C s-1 temperature transition rate while continuously monitoring the fluorescence.

In addition, without heating the template for more conformal coverage by diffusion, a similar effect could be obtained by improving the directionality of evaporated species that we obtained by reducing the evaporating distance between the source and the substrate (template).

Chemical cross-links were reversed by heating the samples overnight at 65°C; the DNA was separated from protein and used as a template for PCR reactions.

Annealing was done by heating the primer templates in a molar ratio of 1 1.4 to 95 °C and then slowly cooling to room temperature.

The heating cycle was initiated by heating the jacket, followed by heating the chamber.

By slightly heating the MCC template before Au film evaporation, the void between three adjacent PS spheres can be controllably shrunk.

Herein, we report an effective strategy for homogeneous and polyporous MoO3@CuO composite by heating a POMs@MOFs template (POMs = polyoxometalates, MOFs = Metal-organic frameworks), in which the Mo-POMs are incorporated into Cu-MOFs as secondary building units.

Input random RNA was generated by T7 in vitro transcription: 1 μg T7 oligo was annealed to 1 μg of RBNS T7 template by heating the mixture at 65° C for 5 min then allowing the reaction to cool at room temperature for 2 min. The random RNA was then in vitro transcribed with HiScribe T7 In vitro transcription kit (NEB) according to manufacturer's instructions.

The end-labelled primer was annealed to each template (primer: template molar ratio of 1 2) by heating the mixture at 70 °C for 4 min, followed by slowing cooling to room temperature over a period of 2 h.

The RNA template was hydrolyzed by heating in the presence of 100 mM KOH followed by neutralization with 1 M Tris HCl pH = 5 (to a final pH = 8), and the resulting cDNA was isolated on a 10% denaturing polyacrylamide gel.

These ingredients are heated, causing the template DNA to separate into two strands.