Cells were imaged either at 30°C, or 36.8°C by heating the microscope objective with a flexible resistive heater (Omega, Stamford, CT) utilizing an on off controller (Minco, Minneapolis, MN), which maintained the temperature at the objective within ±0.1°C as readout by a 100 Ω platinum thermistor (Minco).
Nanocomposite ZnO-TiO2 powders of varying ZnO/TiO2 molar ratios have been prepared from their salt/compound by heating at 600°C and 900°C and characterized using scanning electron microscope and X-ray diffraction techniques.
The use of computerized image analysis techniques allows all the data contained in the images provided by the heating microscope to be transformed rapidly into valuable and highly accurate information.
The MSCs were constructed, for BTS2.5 and BTS15 graded layers in FGMs, using shrinkage data obtained by a heating microscope during sintering at four constant heating rates, 2, 5, 10 and 20 °/min.
The worms were transferred into molten paraffin wax at 56°C (2 times for 10 min each) to fill open cavities with wax, embedded in wax and then cut into 4 μm sections with a microtome (Yamato Koki Individuals. Co., Ltd., Tokyo, Japan).. Worm sections on glass microscope slides were deparaffinized by heating at 50°C for 2 hr and then washed twice in xylene (5 min/wash).
Carbohydrate glasses were prepared by heating to 114+/− 2 °C and placing one drop of molten syrup onto a pre-heated (125 +/− 2 °C) silica glass microscope slide placed on a hot plate (VWR International, Radnor, PA).
Jurkat cells lysed by heating at 56°C lost all bright red mitochondria staining from JC-1, as observed by FACS and by microscope.
According to heating microscope results, pumice was observed deforming at 850 °C and flowing at 1270 °C.
The application of the heating microscope technique for studying the shrinkage behavior of ceramic and glass powder compacts during sintering has been reported by Boccaccini and Trusty [14].
Figure 1 SEM images of colloidal carbon spheres produced by the one-step approach by heating at 180 °C for 4 h under various concentrations:a 1.5 M,b 1.0 M,c 0.6 M,d 0.4 M,e 0.2 M, andf 0.1 M. The morphology and size of the carbon colloidal particles were checked using scanning electron microscope (SHIMADZU, SSX-550, SUPERSCAN Scanning Electron Microscope).
