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The glucosylation reactions were stopped by heating each sample for 20 min at 70 °C.
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For formalin-fixed, paraffin-embedded tissues, the paraffin was first removed with octane methanol and the proteinase K extraction step was extended to 3 days, adding fresh enzyme on each day, followed by heating the sample at 95 °C for 15 min prior to protein precipitation.
Analysis was first performed by heating the sample from 25°C to 450°C at a rate of 2°C/min.
By heating the sample, the contribution from incomplete zeroing was excluded and at the same time the sample was sensitised.
This method uses condutimetric monitoring and induces the degradation of FAAS by heating the sample at a constant temperature.
The reaction was quenched by heating the sample to 96°C for 10 minutes.
Analyses were performed by heating the sample from 50 1000 °C with a temperature ramp of 5 °C/min.
Scientists can read the clock by emptying the traps in the lab, either by heating the sample (TL) or by tickling it with light (optically stimulated luminescence, or OSL).
The analysis was conducted by heating the sample up to 950 °C at a constant rate of 5 °C min−1 under an Ar flow (4.95 % molar H2 in Ar).
These asymmetric dimers can be permanently welded onto single pieces by heating the sample at temperature slightly higher than the glass transition temperature of PS.
The effect of increased temperature on teneligliptin was studied by heating the sample at 69 °C for 48 h in refluxing apparatus.
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