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Spores were purified by gradient separation using RenoCal-76 (Bracco Diagnostics, Princeton, NJ) (6 ).
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Using sterile technique, neutrophils were isolated from whole blood by density gradient separation using Polymorphprep (13.8% sodium diatrizoate and 8.0% polysaccharide, Axis-Shield, Rodelokka, Oslo, Norway), with centrifugation at 500 g for 40 minutes.
Cells were isolated by density gradient separation using LSM 1077 Lymphocyte separation medium and sorted for CD3+ cellsCD14− CD45RO+ cells using FACS.
Cell suspensions were subsequently passed through a 37 μm nylon mesh followed by density gradient separation using HISTOPAQUE 1083 (Sigma-Aldrich), according to the manufacturer's instructions.
PBMCs were obtained from 13 mL of heparinized whole blood of four adult female New Zealand White rabbits (numbered 1, 2, 4, and 5) by density gradient separation using ficoll-histopaque.
Peripheral blood mononuclear cells (PBMC) were isolated by ficoll-hypaque density gradient separation, using standard protocols.
Tumor cells were enriched by density-gradient separation using Percoll (blood) or Ficoll Hypaque (BM) solution.
Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats obtained from healthy donors by density-gradient separation using Lymphoprep™ reagent (Axis-Shield, Dundee, UK).
Afterwards the peripheral blood mononuclear cells (PBMC), were isolated by Ficoll density gradient separation.
When comparing gradient separations using columns of different length, one should also compare different gradients.
Peripheral blood mononuclear cells (PBMCs) were obtained by gradient separation of whole blood using Histopaque (GIBCO-BRL, USA).
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