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To the resuspended DNA, 1/10× volume 3 M sodium acetate and 2.5× volumes 96% ethanol were added, mixed by gentle inversion and incubated at -20°C for 2 hours.
After a 30 min incubation at 37°C, 1/5× volume 5 M NaCl and 1× volume chloroform:isoamyl alcohol (24:1) were added, the tube was mixed by gentle inversion and centrifuged at 18000× g for 20 min.
His-Bind resin was mixed by gentle inversion and was added to a column using a wide-mouth pipette.
Immediately following collection of a given blood sample, it was mixed by gentle inversion and the tube immediately placed at 4°C.
Samples were mixed six times by gentle inversion and stored on ice before centrifugation at 3,200 rpm for 15 min at 4°C within 15 min of collection.
Blood (40 mL aliquots) was collected into 4 mL sodium citrate 3.8% (3.8 g in 100 mL sterile water), mixed by gentle inversion and centrifugation (350 g, 20 min).
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An equal volume of phenol∶chloroform∶isoamyl alcohol (24∶24∶1) was added and mixed, by gentle inversion, for about 10 minutes and centrifuged at 500 g for 10 minutes at 10°C.
The resultant solution was mixed by gentle inversion for 10 s and then left undisturbed overnight.
We mixed the blood by gentle inversion of the tube and centrifuged it at 1,200 × g for 10 min. The plasma supernatant was discarded and the red blood cells (RBCs) were washed 4 times by suspending them in saline (0.9%) before centrifugation at 1,200 × g for 10 min. The final suspension consisted of 5% by volume RBC in saline.
The cuvettes were sealed with Parafilm and mixed by gentle inversion.
Then 20 μL of xanthine oxidase solution (0.90 mU/mL) was added and mixed by gentle inversion.
More suggestions(15)
by gentle stirring and
by gentle mixing and
by gentle persuasion and
by gentle suction and
by gentle scrubbing and
by spatial inversion and
by gentle terrain and
by gentle pipetting and
by gentle tapping and
by gentle heating and
by gentle washing and
by gentle laughter and
by gentle aspiration and
by gentle sonication and
by gentle scraping and
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