The homogenate was filtered through three meshes and centrifuged at 700×g for 10 min. Fractionations of nuclear and plasma membrane; The pellet was resuspended in 120 ml of STEA solution by gentle homogenization, and the resuspension was dispersed in 1080 ml of isosmotic Percoll solution (15.7% Percoll, 0.25 M sucrose, 1 mM EGTA, 50 karikllein units/ml aprotinin and 10 mM Tris HCI (pH 7.5)).
Sporozoites were released from the glands by gentle homogenization and washed three times before enumeration.
Intact chloroplasts were isolated in a semi-frozen 20 mM Tricine-NaOH (pH 7.5) buffer containing 330 mM sorbitol, 15 mM NaCl, 4 mM MgCl2 and 40 mM ascorbate by gentle homogenization of pea and bean leaves.
Nuclei were resuspended by gentle homogenization in 0.88 M sucrose and 3 mM MgCl2 and centrifuged at 2500 g for 20 min to remove cell debris.
Cell nuclei were resuspended in buffer A, centrifuged (1000× g, 10 min, 4 °C) and opened by gentle homogenization with three volumes of buffer A in Dounce homogenizer.
Total RNAs from the ovarian tissues of the serous carcinoma were isolated by gentle homogenization using Trizol®.
The resulting microsomal pellet was resuspended in 1 to 2 mL of TEG50 buffer (50 mM Tris-HCl, 1 mM EDTA, 20% glycerol (by vol)., 1 mM DTT; pH 7.4) by gentle hand homogenization using a Potter-Elvehjem homogenizer and microsomal suspensions were stored at -80°C until use.
After gentle homogenization with a Dounce homogenizer, cell lysates were centrifuged at 1000×g for 5 min to remove unbroken cells and nuclei, and then cytosolic fractions and the mitochondrial pellets were obtained by further centrifugation at 10,000×g for 30 min. Data were analyzed as means±SD.
After gentle homogenization with a Dounce homogenizer, cell lysates were subjected to differential centrifugation.
Stomaching is considered as a gentle homogenization method since heat development is marginal and the peristaltic movements of the paddles distribute the input energy on a large area, reducing potential peaks in shear forces.
Mesophyll cells of Zinnia were isolated under sterile conditions from the first true leaves (3rd leaf pair just emerging) of 14-day old seedlings, by gentle mechanical homogenization of surface-sterilized leaves in a cultures medium following the protocol of Fukuda and Komamine [ 30] with modifications for increasing the yield of produced TEs [ 33].
