Sentence examples for by gentle centrifugation and from inspiring English sources

Spheres were collected by gentle centrifugation and dissociated with trypsin-EDTA.

Cells were harvested by gentle centrifugation and gently resuspended in labeling buffer.

After 5 days, mammospheres were collected by gentle centrifugation and disaggregated with a Non-enzymatic Cell Dissociation Solution (Sigma-Aldrich).

The next morning, the beads were collected by gentle centrifugation and the supernatant (unbound fraction) transferred to a fresh tube for subsequent analysis by mass spectrometry.

The spheres were collected by gentle centrifugation and trypsinized to single cells in Trypsin-EDTA solution as well as the adherent cells.

Cells were collected by gentle centrifugation and then lysed with 1% Triton-X100, 0.1% SDS, 1% 0.1 M sodium orthovanadate and protease inhibitor cocktail (Roche Diagnostics) dissolved in PBS.

After 7 days in culture primary cervospheres were collected by gentle centrifugation, dissociated, sieved, and re-plated at low density in order to give rise to secondary spheres.

For serial passages, mammospheres were collected by gentle centrifugation (800 rpm) and dissociated enzymatically (10 minutes in 0.05% trypsin containing 0.53 mM ethylenediaminetetraacetic acid) and mechanically using a fire-polished Pasteur pipette as described elsewhere.

Following sedimentation of the tumor spheroids by gentle centrifugation, supernatants were aspirated, and solid tumor spheroids were disintegrated by enzymatic digest.

The bead-labeled cells were concentrated in ~∼0.5 ml PBS by gentle centrifugation (5min, 2500 ×g) and 2 × 10 cells were loaded onto the magnetized aging column.

After 10 days in culture, mammospheres were collected by gentle centrifugation (200 × g) and agitated with a P200 micropipette to segregate individual spheres to split for embedding or secondary cultures.