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After dissolution, cells were recovered in high yield and with high viability by gentle centrifugation.
Then, the pigment layer is removed by gentle vacuum aspiration with a 25 gauge cannula.
The products with black color were then precipitated by gentle centrifugation.
All the media were discarded after 96 h by gentle pipetting.
Then, NMP was added into the mixture, followed by gentle grinding to generate a homogeneous slurry.
The resultant solution was mixed by gentle inversion for 10 s and then left undisturbed overnight.
The bulk rock was disaggregated by gentle crushing and suspended in distilled water.
The biofilm cells were removed from the surface by gentle sonication for 1 min.
Non-rhizosphere soil was removed by gentle shaking, leaving behind only the rhizosphere soil.
However, the studied area is characterized by gentle dipping (to the north) bedrock of carbonate lithology.
Samples were obtained by gentle scraping with a sterile plastic implement.
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