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To reveal the alcohol tolerance mechanism of SY1043, we investigated the relationship between alcohol tolerance and four mutations found in SY1043 by genome resequencing analysis.
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In our genome resequencing analysis, the genomic regions with low read coverage (less than 10 reads) were excluded from the mutation identification procedure.
Genome resequencing analysis of IT182932 was reported by Kim et al. Raw reads of 39.8 Gb of the IT182932 genome produced by Illumina-GA were re-analysed in this study.
However, accurate genetic changes occurred in CIBTS0735 remain to be elucidated by genome resequencing and transcriptome analysis and this work will be pursued in our following research.
Choi, J. et al. Whole-genome resequencing analysis of Hanwoo and Yanbian cattle to identify genome-wide snps and signatures of selection.
Whole-genome resequencing analysis of the tolerant strains will provide information on the mutations that caused the observed phenotypic changes during adaptive evolution.
Recently, an international initiative, i.e., "The 3000 rice genome sequence project" has been completed to generate 20 million SNPs by genome resequencing of 3000 rice genotypes (Alexandrov et al. 2015).
The program uses single nucleotide polymorphism (SNP) data in a given genomic region as input dataset, the region of which would be as large as the whole genome obtained by genome resequencing.
SNP genotyping eliminates the need to identify viable marker features via permutation testing, relying instead on SNPs identified by genome resequencing methods.
Estimation of phylogenetic relationships among Medicago species by genome resequencing has been reported [ 42].
We identified the mutations appearing during the experiment by genome resequencing at three late time points during the experiment.
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