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The newly predicted β-glucoside BglT transporter was experimentally confirmed by genetic complementation in E. coli Δ bglF mutant (a knockout of cellobiose PTS system).
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Finally, the most promising candidates are tested by genetic complementation or epistasis in transgenic animals.
The growth rate increments observed for the luxN, luxPQ and luxS mutations were confirmed by genetic complementation, indicating a role for these genes in affecting Vh growth rate by an unknown mechanism.
Sequencing of the entire gene from two DEB-derived mutants D1137 and D2943 (confirmed to be spl1 alleles by genetic complementation [ 25]) also showed single nucleotide polymorphisms (SNPs) in the Os12g16720 gene model.
This is indicated by genetic complementation analyses, demonstrating that neither abp1-1 nor abp1-1s complement segregation of bsm1-1 embryo-lethal phenotypes, when analyzing progeny of crosses.
The defined expression level in independent transfectants will be advantageous for studies in which the effect of amino acid alterations is analyzed by genetic complementation.
The identity of the SMG11 gene was further confirmed by genetic complementation analysis.
All phenotypic changes observed could be reversed by genetic complementation, with exception of those seen for the ptb mutant.
Loss of one GLOI allele by gene disruption was compensated by genetic complementation with an episomal copy of GLOI.
Using isogenic strains and confirmation by genetic complementation, it was found that the absence of an active luciferase pathway increased Vibrio harveyi's growth rate by ca. 30%.
Functional expression of GGPP synthase was investigated by genetic complementation with the carotenogenic crt gene cluster.
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