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This area also holds tremendous potential for discovering novel relationships between genotype and phenotype by generating genetically engineered animal models with alternatively spliced gene variants.
This effect has been achieved by generating genetically engineered "hypoallergens" that retain their ability to stimulate T cells but that have reduced IgE antibody binding capacity.
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Our study goes beyond cultured cells and shows the utility of the Cas9/RNA as a genetic tool in generating genetically engineered mice and, potentially, other mammals.
The toolbox for generating genetically engineered mouse models (GEMMs) has been steadily growing over the last couple of years.
The N-glycosylation sites of CXCR4 were altered by amino acid exchanges to generate genetically engineered mutants of CXCR4 with and without N-glycosylation sites.
We first generated genetically engineered MVs by expressing high levels of the suicide gene mRNA and protein cytosine deaminase (CD) fused to uracil phosphoribosyltransferase (UPRT) in MV donor cells.
Moreover, a common practice is the use of embryonic stem cells from the 129 mouse strain to generate genetically engineered mice in which germ transmission can be scored by a chimeric fur.
Here we first generated genetically engineered mice carrying a loxP-flanked (floxed) jsap1 gene.
Already, scientists have used it to generate genetically engineered monkeys, and the technique has stirred debate over whether modified humans are next (see "Engineering the Perfect Baby").
We have overcome many of the obstacles of transplantable and carcinogen-induced models of cancer by generating autochthonous, genetically engineered mouse models (GEMMs) of cancer that can be induced to express or lack model tumor-specific neo-antigens (TSAs) to investigate the consequences of immune-tumor interactions in the pathophysiology of distinct forms of cancer.
We have generated several genetically engineered AB1 cell lines in which both alleles of the CRABPI gene have been deleted by homologous recombination.
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