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We have therefore adopted the BL31 cell line as a platform for comparing the function of various EBV proteins, by generating cell lines infected by various EBV mutants.
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We modelled the disease here by generating stable cell lines expressing disease-associated mutants.
We further substantiated the rescue experiment just described by generating ZHBTc4 cell lines maintained by wild-type Oct4 and, separately, by Oct4λVP2 and by Xlpou91λVP2.
To address this concern, we have begun by generating a cell line that consistently and stably expresses the EP1 receptor and could be used as a sensitive drug target.
We perturbed CCL2 expression in Colon-26 cells by generating Colon-26 cell lines with stable short-hairpin RNA (shRNA) knockdown of their endogenous Ccl2 and shRNA for GFP (shControl) as a control.
Both authors suggest a role for AR signaling in this subtype of breast cancer based on comparison to data generated by cell lines.
The procedure for generating stable cell lines by retroviral vectors or lentiviral vectors is complicated with a stringent specification for the materials and equipments.
We confirmed the RNAi phenotype by generating FAT1 mutant cell lines by CRISPR/Cas9-mediated genome engineering.
SPECT/CT imaging revealed intense 99mTcO4 – uptake by the subcutaneous xenografts generated from cell lines A, B, and C, but not by those generated from cell line N (Figure 2C).
Government-financed researchers are forbidden by Congress to generate such cell lines because the blastocyst is destroyed, but were allowed by President George W. Bush to work on lines already in existence as of Aug. 9, 2001.
We investigated this possibility, by generating stable MM cell line-derived clones expressing a cyclin D1-green fluorescent protein (GFP) fusion protein (D1-GFP) or GFP alone.
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