Sentence examples for by generating a standard from inspiring English sources

Reaction efficiencies were between 94 and 105% for all primers, calculated by generating a standard curve and plotting the threshold cycle (CT) against the logarithm of four known tomato DNA dilutions.

Linearity was verified by generating a standard curve using a dilution series of the indicated sample.

Real-time PCR products may be quantitated by generating a standard curve, or quantitated relatively to a control gene.

The real-time PCR assay was made quantitative (qPCR) by generating a standard curve from the tenfold serial diluted and enumerated spore suspension of C. botulinum.

Northern Blotting was performed by generating a standard 13% polyacrylamide gel, which was loaded with V. proteolyticus and E. coli 5S rRNA in various calculated ratios.

Quantitation of calprotectin within the serum samples was calculated by generating a standard curve with recombinant human calprotectin that was provided in the kit.

The fluorescence was normalized to AMC levels by first generating a standard curve for AMC fluorescence.

Quantification was performed by first generating a standard curve of primer amplification efficiency, using whole-gut cDNA from colony B1#1 with a five-fold dilution series, and then extrapolating the experimental samples onto the curve.

These peaks of known fluorescence intensities are converted to Molecules of Equivalent Soluble Fluorochrome (MESF) units, using a proprietary spreadsheet provided by Bang Laboratories, Inc., generating a standard curve.

To quantify the serum virus load, cDNA from cultured PRRSV (with a known TCID50) was serially diluted by tenfold to generate a standard curve.

(A ) Silver-stained gel image of purified V. fischeri LPS separated by SDS-PAGE used to generate a standard curve by densitometry.