Coverage of the genome sequence by RNA-seq transcripts was determined by generating a "base pair map" of the.bam alignment using the bam2depth function in SAMtools (http://samtools.sourceforge.net) [ 42].
Native contacts are defined by firstly, tabulating all intermolecular donor-acceptor atom contacts shorter than a center-to-center distance dmax (4.5 Å) in the x-ray structure of the protein complex and secondly, by generating a table listing dependent pairs of contacts, i.e. those contacts in which either atom is within dmin (<6 Å) of an atom of another contact within the same protein.
The E. coli MC1061 ϕ24B) genome was obtained through whole-genome shotgun pyrosequencing by generating a standard DNA mate-pair library with an 8-kb insert size, using a 454 preparation kit (Roche Applied Sciences, Indianapolis, IN).
More precisely, we generated sequence pairs by generating a first sequence using a Bernoulli model with probability 0.25 for each nucleotide.
In mid-June, China set a new world record for quantum entanglement, by generating and transmitting a pair of particles—"that behave like twins and experience the exact same things simultaneously"—to ground-based locations 1 200 km apart, from a satellite almost 500 km above the earth.
To call nucleosome positions, the first base pair of each read was added and the Watson and Crick reads were combined in order, first by chromosome and then by base pair, generating a tab delimited file with chromosome, base pair, F/R, and count.
Chains of magnetic endosomes were aligned along a homogeneous horizontal magnetic field of 76 mT, generated by two permanent magnets, and were made to oscillate by applying an additional field generated by a pair of perpendicular coils (each 6mH inductance) supplied with a sinusoidal current.
Electron spin-dependent transport properties have been theoretically investigated in two-dimensional electron gas (2DEG) modulated by the magnetic field generated by a pair of anti-parallel magnetization ferromagnetic metal stripes and the electrostatic potential provided by a normal metal Schottky stripe.
It is basically an optimization method that starts from an initial estimate of T and iteratively refines it by generating pairs of corresponding points on the scans and minimizing an error metric, e.g., the sum of squared distances between corresponding points.
The recovery cycle of the Masseter Inhibitory Reflex (rcMIR) was studied by generating pairs of stimuli with identical characteristics, performed percutaneously with a bipolar electrical stimulator positioned on the patient's face in the area of the mental nerve.
