Sentence examples for by generating a mutant from inspiring English sources

We achieved premature expression of the middle genes (NDT80, CLB5, and DIT1) by generating a mutant expressing Ndt80, their transcriptional activator, from the IME2 promoter (Figure 6A).

One way this could be achieved would be by generating a mutant form of Robo insensitive to Comm sorting, but still capable of transmitting the repulsive Slit signal in vivo.

We used a similar strategy to further test for a role of active nuclear transport in control of the steady state levels of EGFP-LC3 in the nucleus versus cytoplasm, by generating a mutant form of EGFP-LC3 (EGFP-LC3 mNES) in which three leucines in the putative NES were mutated to alanine (L63A L63A L63A).

In the present work, we have addressed this question by generating a mutant form of gal-7 (R74S) with altered subcellular localization and tested its ability to mediate resistance of breast cancer cells to drug-induced cell death.

demonstrate that this mutation can be rescued to birth by generating a double mutant with Mavs, an innate immune gene, indicating the central role ADAR1 plays in innate immunity.

We may be able to overcome this issue by generating a CASP3* mutant that does not interact with CIAP.

Remarkably, the phenotypes of the Bpnt1 knockout are rescued by generating a double mutant mouse deficient for both PAP synthesis and hydrolysis, consistent with a mechanism in which PAP accumulation is toxic to tissue function independent of sulfation.

Here, we examined the role of PPK2 in C. jejuni patho-physiology by generating a ppk2 mutant in a highly virulent strain of C. jejuni.

We did this by generating a cysteine mutant of a nearby residue located in domain 4, Glu712Cys.

Here, we further investigate the functions of protein phosphatases in A. fumigatus by generating a null mutant collection for the phosphatase catalytic subunit encoding genes.

Here, we test this sensitivity by generating a Pin1 mutant, I28A, which weakens the wild-type interdomain contact while maintaining the overall folds of the two domains.