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In patients undergoing living donor liver transplantation (LDLT), expression of A20 also significantly increased 1 hour after reperfusion, as determined by gene microarray analysis, validating the fact that A20 is part of the regenerative response in humans [20].
How do the new NGF-regulated genes identified by gene microarray analysis contribute to the control of sympathetic neuron death and survival?
A detailed characterization of the molecular mechanism evoked by exposure of human skin fibroblast cells to 0.6 and 0.06 μg MWCNTs/mL was evaluated by gene microarray analysis.
To further address the molecular mechanism involved in the enhanced sensitivity of androgen withdrawal in LNCaP/IL-6 1, the infLNCaP/IL-6 1-6 overexpression on global gene expression was assessed by gene microarray analysis.
Quantitative PCR was used to examine the expression status of the three candidate genes which have statistically differential expression in PCa tissues compared with non-cancerous prostate tissues detected by gene microarray analysis.
Although it would be necessary to perform functional analysis to identify genes having potential functions in the mechanism described above, several candidate genes could be identified by gene microarray analysis.
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Gene lists identified by single gene microarray analysis were compared using the FatiGO web application from BABELOMICS v3.1 ([28], [28], http://www.babelomics.org).org
A first step in this was taken by our laboratory by performing gene microarray analysis and real-time q-PCR on rat bladders at various time points following SCI [10].
In comparison, the genes Wnt3 and Wnt7a, which were found to be up-regulated by gene expression microarray analysis, were validated as up-regulated by real-time, RT-PCR and western blotting in SmoA1 +; Pten +/− medulloblastomas (Figure S1).
This analysis is particularly important for upstream regulators that are regulated at the protein level (e.g., by phosphorylation, or by ligand binding) and therefore would not be identified by gene expression microarray analysis.
Previously in an effort to understand FLCN function, we searched for differentially expressed genes in cells expressing mutant FLCN compared to wildtype FLCN by gene expression microarray analysis.
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