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Edges can then be directly weighted by gene expression values.
cgene expression values of MAPwt TPEN culture divided by gene expression values of MAPwt standard culture from RNA-sequencing.
The functional activities of genes, as measured by gene expression values, reflect the interplay of the genes and their products in the underlying gene network.
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These are followed by case-by-case gene expression values (illustrated as z scores) which are broken down into components identified by coloured bars on the right of each heatmap.
Data was normalized by transforming the data by lg10 (gene expression values).
For example, a set of 100 patients each characterized by 5000 gene expression values is still represented by a 100 x 100 kernel matrix [59].
For example, a set of 100 patients each characterized by 6,913 gene expression values is still represented by a 100 × 100 kernel matrix [ 40].
Initially, we attempted two normalization methods applicable for both Agilent and Affymetrix array types analyzed in our studies: i) RMA background correction [ 46] followed by quantile normalization with [ 47]. and ii) normalization by ACTB gene expression values.
For each simulation model, we also calculated the proportion of probes associated with the phenotype and R2, the proportion of variation in the quantitative phenotype explained by the gene expression values in the pathway.
Using two gene expression data sets that compared MSI with MSS colon cancers using the same Affymatrix chip (PMID: 17047040, GEO entry GSE4554[31], PMID: 19088021, GEO entry GSE13294[32]), we ranked shortlisted genes (NMD-resistant transcripts, ≥8 mer cMS, ≥10 amino acids of novel mutant C-terminus) by median gene expression values.
Probeset expression specificity was calculated according to [ 60] by standardising gene expression values within, and then between microarray assays.
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