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Interestingly, only one Egf receptor family member, Erbb3 [ 51], was found by gene expression array and qPCR analysis to be differentially expressed in the normal mammary epithelium.
Cardiac molecular pathways influenced by α1A-AR-AB signaling were investigated by gene expression array analyses.
Finally, we monitored endorgan damage with echocardiography, invasive hemodynamics, histological analysis, radiotelemetrically determined blood pressure measurements, and by gene expression array.
There was a good and statistically significant correlation between the magnitude of altered expression measured by gene expression array and that measured by RT-PCR for these genes (Figure 1).
A more precise measurement of transcript abundance of these genes was performed by qRT-PCR, which revealed that differential expression was significantly greater than had been detected by microarray: the range of fold difference between ALL and AML by gene expression array was 1.0 2.8 and by qRT-PCR 2.3 45.6 (Figure 3B).
By gene expression array analysis, we found that forced expression of WT1 had a regulatory function on many known WT1 target genes.
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We found that DNA methylation and H3K9 acetylation distinguished these leukemias of distinct cell lineage, as expected, but that an integrative analysis combining the information from each platform revealed hundreds of additional differentially expressed genes that were missed by gene expression arrays alone.
KLF3A was identified as a susceptibility gene for childhood asthma by gene expression arrays in nasal cells and further studies found that genetic variation in KLF3A is associated with asthma.
Unfortunately, the low expression levels of TERT may impede detection by gene expression arrays.
To confirm Notch pathway activation, a profile of genes upregulated following release from gamma-secretase inhibition was characterized by gene expression arrays.
We then profiled by gene expression arrays both the transcriptome and the translatome, before and after 40 minutes of EGF treatment.
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