We recently developed a method to connect miRNAs to biological function by comparing miRNA and gene array expression data from the NCI60 cell lines without using miRNA target predictions (miRConnect).
The studies presented here provide additional information to the study that examined C/EBPα expression by gene array [ 14] and in which expression of C/EBPα RNA was increased by three fold in cancerous prostate epithelium versus normal epithelium.
Analysis of apoptosis-related gene expression by gene array also demonstrated that NRP-1 overexpression was associated with induction of the antiapoptotic regulator, MCL-1.
In addition, PROK1 has been demonstrated by gene array analysis to regulate endometrial expression of a host of genes known to be important for implantation, including cyclooxygenase-2, heparin-binding epidermal growth factor (EGF), Dickkopf-1, interleukin (IL -11, and leukemIL -11ibitory fandor (leukemia.
The present findings demonstrating a similar set of proteins accumulate in muscle fibers in this diabetes model as in other muscle disease conditions discussed above, is also supported by gene array studies that examined global gene expression changes in different models of muscle disease.
We employed a two-step quantitative RT-PCR to validate expression changes identified by gene array analysis for the three selected genes in all 49 cases.
This is one of the largest available datasets measuring the overall reproducibility of gene expression data measured by gene arrays and demonstrates sufficient reproducibility.
We employed the connection between gene expression, provided by gene expression array, and TFBS that contain genetic variations, identified by SNP array, to associate two types of arrays.
There was a good and statistically significant correlation between the magnitude of altered expression measured by gene expression array and that measured by RT-PCR for these genes (Figure 1).
There was a good and statistically significant correlation between the magnitude of altered expression measured by gene expression array and that measured by RT-PCR for these genes.
