Sentence examples for by gel separation using from inspiring English sources

For example, in proteomics, relative quantification was obtained by gel separation using densitometry or radiolabelling.

In the present study, we narrow down the spectrum of potential protein candidates by proteomic analysis of inhibitory protein fractions prepared by CM and HighQ column chromatography followed by BN/SDS/SDS PAGE gel separation using Nano-HPLC-ESI-Q-TOF mass spectrometry.

The GAGs' composition in vertebral body GP was resolved by separation using gel electrophoresis in 1% agarose gel [ 29] using 50 mM barium acetate buffer at pH 5.0.

The purity of the compounds was accomplished by column chromatographic separation using silica gel as the stationary phase and chloroform:methanol (9 1) as the mobile phase.

The commonly used techniques to discover these biomarkers, also referred to as quantitative proteomics, are performed by protein separation using either two-dimensional gel electrophoresis (2DE)- or liquid chromatography (LC -based methods coupLC -basedprotein identification using methodsecoupledry (MS) [ 10].

Molecular weights of the extracted proteins were determined by electrophoresis separation using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Laemmli, 1970).

Briefly, gels were lysed in denaturing Laemmli buffer followed by protein separation using SDS-PAGE.

The pI of the various AP isozymes was determined by separation using Invitrogen Novex® pH 3 7 IEF Gel (Life Technologies, Paisley, UK).

Molloy, M. P. et al. Extraction of membrane proteins by differential solubilization for separation using two‐dimensional gel electrophoresis.

In general, the classical proteomics workflow includes protein separation using gel-based or gel free techniques followed by the identification by mass spectrometry.

Size selection (300 600 bp) was performed by agarose gel separation and followed by gel purification and PCR amplification.