Sentence examples for by gel purification using from inspiring English sources

All PCR products were pooled and precipitated under vacuum (SpeedVac; Heraeus Instruments, Hanau, Germany) followed by gel purification using the Qiaquick PCR purification kit (Qiagen, Valencia, CA, USA).

Fragments <120 bp (β-lactamase) or <300 bp (fluorescence proteins) were isolated by agarose gel purification using QIAEX II (Qiagen, Valencia, CA).

Bands ≥10 kb in size were selected by excision to exclude any possible L1 cDNA and subjected to gel purification using a Qiagen gel purification kit (#28606).

The gDNA inserts of PGB02 and PGB04 were isolated by gel purification and sheared using a nebulizer (Invitrogen).

Shearing using AFA, followed by gel purification, allows us to generate unbiased cDNA tags at a user-specified distance from the poly(A) site for mapping to the transcriptome.

Amplicons were purified by agarose gel electrophoresis followed by purification using the MiniElute Gel Extraction kit (QIAGEN, Valencia, CA, USA), then sequenced on an automated Applied Biosystems 3730 system (Foster City, CA, USA) using cycle sequencing dye terminator chemistry.

i. Confirm viral insert identity by sequencing using M13F and R primers and REDTaq polymerase, followed by gel purification and sequencing of PCR product.

MP98 was deglycosylated (DMP98) by treatment with trifluoromethanesulfonic acid using a commercial kit (GlycoProfile IV chemical deglycosylation kit, Sigma) followed by gel purification, as in previous studies [21].

The primer-annealed DNA templates were separated from excess labeled primer or remaining template DNA on 8 % non-denaturing polyacrylamide gel followed by gel-purification and used in a primer extension assay by Taq DNA polymerase.

Lipid-free polysaccharide (PS) was prepared by treatment of the LPS with dilute acetic acid followed by purification using gel permeation chromatography [ 31].

ClavMO was produced in a 5 L scale followed up by purification using gel filtration.