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In early 3C experiments, ligation products were quantified by gel image analysis, which is error-prone.
Relative expression ratio was obtained by gel image analysis, which was performed with PDQuest version 8.0.1 (Bio-Rad, Hercules, CA, USA).
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Protein raw extracts of labeled and unlabeled cells were either analyzed separately by 2-D gel image analysis or 1∶1 mixed and further separated via 1-D PAGE (Figure 1).
Several types of image filtering algorithms are used by 2-D gel image analysis software to remove background and noise (Fig. 6).
The proteins from the control group and MeJA-treated plants were compared by using 2-D gel image analysis.
Bands were visualized using an enhanced chemiluminescense detection kit (Pierce Co .. Quantification of bands was done by gel densitometry with gel image analysis system (American UVP Co).
Immunoreactivity was detected by using the ECL detection system and semi-quantified with gel image analysis software.
To improve 2D gel image analysis results, protein spot quantitation was optimized by missing value imputation and normalization.
The amplified PCR products were electrophoresed on a 1.5% agarose gel and visualized by RedSafe Nucleic Acid Staining solution (Intron Biotechnology, Sungnam, Korea) using the i-MAX gel image analysis system (CoreBioSystem, Seoul, Korea).
PCR products were analysed by electrophoresis on 1.5% agarose gels, and the size analysis of PCR fragments was performed using the Gel Image Analysis System (Tanon2500, Shanghai, China).
Digital gel image analysis using Phoretix 1D software.
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