Sentence examples for by gel filtration using from inspiring English sources

UppP was further purified by gel filtration using a Superdex 200 Increase 10/300 GL column equilibrated with 20 mM HEPES pH 7.0, 150 mM NaCl, 0.016% DDM before being concentrated to 12 mg mL−1 in an Amicon Ultra-15 concentrator (Millipore) with a molecular weight cutoff of 50 kDa.

Excess NEM in the supernatant was removed by gel filtration using PD-10 columns.

The molecular mass of the native enzyme was estimated by gel filtration using a TSK-GEL column.

The supernatant was analyzed by gel filtration using the FPLC system as described above.

Excess biotin and salts were separated by gel filtration using D-Salt™ dextran desalting columns (Pierce™).

Purification of free 99mTc from labeled adenovirus virions was performed by gel filtration using a Sephacryl S-200 column.

The products were desalted by gel filtration using MultiScreen-HV 96-well plates (Millipore), loaded with Sephadex G-50 (Sigma).

Labeled antibodies were purified from free dye by gel filtration using a column provided in the kit.

Small molecules were removed from P. chrysogenum crude extracts by gel filtration using a PD-10 column.

Transcription products of HIV-1 RNA (4.9 kb) were purified with spin columns MicroSpin™ G-25 Columns (American Biosciences), followed by gel-filtration using NAP-5 columns, (American Biosciences) and were used in the in vitro RT assay.

Desalting of purified MPEG-oligonucleotide was carried out by gel-filtration using a 20 x 100 mm column of Sephadex G25-F resin.