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Quantification of PCR products was achieved by gel densitometry using ImageJ software (http://rsbweb.nih.gov/ij/), normalizing against Ornithine Decarboxylase (ODC) expression.
The amount of p62 in the supernatant and pellet fractions was measured by gel densitometry using the ImageJ software.
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Equal amounts of lysate were loaded on an SDS-PAGE gel and quantification of radiolabeled methionine incorporation of lysates was done by gel densitometry (N = 2, SD) using ImageJ.
For each biopsy sample, mtDNA was quantified relative to nuclear DNA (mtDNA content) by qPCR, mtDNA deletions were investigated by long-template PCR followed by gel densitometry, and mtDNA oxidative damage was quantified using a qPCR-based assay.
Stored images of the gels were analysed by densitometry using Gel Base/Gel Blot software.
The PCR products were run on 1.5% agarose gel and the density of the bands on the gel was quantified by densitometry using Tocan gel imaging analysis system.
Bands were visualized using an enhanced chemiluminescense detection kit (Pierce Co .. Quantification of bands was done by gel densitometry with gel image analysis system (American UVP Co).
Protein purity and quantity were analysed on Coomassie blue-stained SDS-PAGE and quantified by gel densitometry.
The purity of heterodimers was measured by gel densitometry (described in Cosedimentation and Densitometry).
The intensity of PCR-amplified bands was determined by gel densitometry (Image Master; GE Healthcare).
The bands were quantified by gel densitometry (Bio-Rad, Hercules, USA).
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