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Affinity purification of recombinant proteins has been facilitated by fusion to a modified protein splicing element (intein).
The fusion protein expression can be further improved by fusion to a mini-intein, i.e. an intein that lacks an endonuclease domain.
We present a method of labeling and immobilizing a low-molecular-weight protein, calmodulin (CaM), by fusion to a larger protein, maltose binding protein (MBP), for single-molecule fluorescence experiments.
We found that new proteins could be recruited to the shells by fusion to a predicted targeting peptide sequence, setting the stage for the use of these remarkably homogeneous shells for applications such as three-dimensional scaffolding and the construction of synthetic BMCs.
Different strategies are used for the delivery of functional proteins such as microinjection, electroporation, liposomes or by fusion to a protein transduction domain (PTD).
Hence, to make neurotrophins transportable across the BBB, chimeric peptides are made in which a neurotrophin is reformulated by fusion to a transport vector.
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We previously reported that the immunogenicity of Hcβtre, a botulinum neurotoxin A (BoNT/A) immunogen, was enhanced by fusion to an epithelial cell binding domain, Ad2F, when nasally delivered to mice with cholera toxin (CT).
Inducible transgene expression was based on post-translational regulation mediated by fusion to an FKBP (FK506 binding protein -based domain targeting the protein -basedidomaineosomal degradatargeting
Our previous results showed that three extracellular domains of human TRAIL can be fused by peptide linkers to yield highly active single-chain TRAIL (scTRAIL) that can be further endowed with target-dependent activity by fusion to an antibody derivative.
The autophagy system facilitates degradation of the cytoplasm following engulfment in a vesicle followed by fusion to lysosomes, a process necessary for both cell differentiation and response to starvation.
Hypothetically, a network can be constructed by pulling tubules out of existing membrane compartments and then connecting these tubules by fusion to give a lattice; by repeating this process, a network can be created.
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