Sentence examples for by fusing the mitochondrial from inspiring English sources
Mito-YFP plasmid was constructed by fusing the mitochondrial target sequence of human COX8A with the YFP sequence.
Mito-DsRed plasmid, a gift from Dr Chen Quan (Institute of Zoology, CAS), was constructed by fusing the mitochondrial target sequence of human TXN2 with the DsRed sequence.
Specific localization is achieved by fusing the FP gene to the mitochondrial targeting sequence of an endogenous protein.
However, when the YFP fragments were separated to different cellular compartments by fusing the N-YFP to the mitochondrial leader sequence cytochrome oxidase subunit-VIII (mito N-YFP HA), the levels of the YFP signal were significantly lowered.
Studies of the fusion dynamics of different mitochondrial sub-compartments, inner membrane (IMM), outer membrane (OMM), and inter-membrane space (IMS) have been achieved by fusing the photoactivatable FPs to the appropriate targeting signals or proteins.
To verify the function of this gene, a chimeric gene was constructed by fusing orf220 with a mitochondrial-targeting sequence derived from atp2-1 amplifiedfrom from tobacco (Nicotiana plumbaginifolia).
We constructed a mitochondrially targeted ICUE3 (MitoICUE3) by fusing ICUE3 with the full-length SOD2, a bona fide mitochondrial protein, to ensure the efficient mitochondria targeting.
We re-engineered the drug resistance genes to express proteins targeted to the mitochondrial matrix and confirmed the location of the proteins in the cells by fusing them with GFP and by Western blot and mitochondrial content mixing analyses.
Mitochondrial morphology was analyzed by transfecting cells with the pDsRed2-Mito plasmid to express the DsRed2 protein fused to the mitochondrial targeting sequence of cytochrome c oxidase.
GFP-aequorin hybrid gene (G5A) fused to the mitochondrial matrix targeting sequence of COX VIII [18] (Figure 1A), was cloned into the pENTRY vector (Invitrogen, CA).
By fusing with stationary mitochondria, a less abundant motile mitochondrial pool regulates stationary mitochondrial turnover and distribution [ 37, 62].
