Sentence examples for by fusing the ends from inspiring English sources

Fusions with fluorescent proteins are usually created by fusing the ends of two coding sequences.

The genetically encoded P48 sensor was created by fusing the CyPet gene to the 5′-end of the FN3BN+I75A gene using a linker that translates to GGSGG.

The DEX-inducible VP16-Fezf2 construct was made by fusing the VP16-Fezf2 protoin thethe 3′-end of human glucocorticoid receptor (hGR) using XbaI- NotI restriction sites (Ryan et al., 2004).

To ensure that differences in FRET are caused by mechanical tension across the biosensor and are not a result of changes in the microenvironment (such as pH, temperature, etc)., we emphasize the need to use a zero-force control, which can be easily generated by fusing the tension sensor module to either end of the POI (Fig.  3a, X-TS0).

We created a genetically encoded variant of the P48 sensor by fusing the CyPet and YPet genes to the 5′-ends of the FN3BN+I75A and P48 genes, respectively.

The CheA-mTurquoise2 (mTq) C-terminal fusion was made by fusing the cheA gene with the fluorophore at the 3′ end using the GTGGSGGS encoding flexible linker.

Here, we establish LNP-directed transfer of the piggyBac DNA transposase protein by fusing the transposase to the integrase protein in the C-terminal end of GagPol.

The SNAP-β1 construct was generated by fusing the SNAP-tag (New England Biolabs, Ipswich, MA, USA) to the N-terminal end of the wild-type human β1-adrenoceptor.

Further examination revealed that the chromosomes were clumping together by fusing at the ends, thereby stretching the cell.

The fiber was sealed on both ends using an arc-fusion splicer to fuse the ends by localized melting.

Strains expressing a C-terminally YFP-tagged version of Chs8, Chs2S222A and Chs2S222E were constructed by fusing YFP to the 3′ end of the remaining CHS8 allele in CHS8/ chs8Δ 0 (NGY609; Table 1) or of the phospho-mutant alleles of CHS2 in chs2S222A and chs2S222E (NGY604 and NGY605; Table 1) using the method described in Lenardon et al. (2007).