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A fusion protein DAMP4var-pexiganan was formed by fusing a variant of DAMP4 protein and an antimicrobial peptide pexiganan.
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By fusing natural yeast variants of pCUP1 with ZeoR, we show here that their different noise levels at equal mean expression indeed provide distinct abilities to survive in constant environmental stress, even if the noise difference is far less important than in Blake's study.
Antibody fragments were also fused to a variant alkaline phosphatase gene with enhanced catalytic activity encoded by a D153G and D330N mutation [ 6, 7].
A second fusion protein was comprised of a bacterial pelB leader peptide fused to a variant form of Galleria mellonella apoLp-III residues 1 91.
First, a human anti-peptide library was constructed by diversifying a scaffold: the human variable heavy chain (VH) germ line gene 3-23, wasch was fused to a variant of the human variable light chain (VL) germ line gene A27, in which L1 was modified to encode the canonical structure found in anti-peptide antibodies.
ArcLight is comprised of the VSD from the Ciona intestinalis voltage-sensor containing phosphatase (Ci-VSP) (Murata et al., 2005) fused to a variant of GFP.
Toward this goal, single-chain FokI TALEN variants have been developed by fusing two FokI monomers with a polypeptide linker that is then fused to the TALE domain (Sun and Zhao 2014).
pCEP4 expression vector (Invitrogen) was modified to facilitate the construction of rabbit Fc fusion proteins by fusing sequences encoding HrTCTP and its variants, Del-N11HrTCTP and Del-N35HrTCTP, to the Fc region of rabbit IgG heavy chain.
We created a genetically encoded variant of the P48 sensor by fusing the CyPet and YPet genes to the 5′-ends of the FN3BN+I75A and P48 genes, respectively.
He produced a giant larva by fusing two embryos.
A marker for the chloroplast envelope inner membrane was generated by fusing A. thaliana plastidic nucleotide transporter 2 (Nto2) thethe N-terminus of the monomeric red fluorescent protein (RFP) variant, mCherry.
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