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Nevertheless these studies may need to be validated by further testing with a small cadaveric bone sample in order to produce conclusive results.
However we do recognise that no synthetic material can be considered as a definitive substitute for bone, therefore studies performed with artificial bone substrates may need to be validated by further testing with a small bone sample in order to produce conclusive results.
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Although the effect of the treatment dose was highly significant, the LD estimates (LD99, 44.1 ± 15.3 Gy, 95% CI) should be treated with caution until this is confirmed by further tests with lower control mortality.
Therefore this material can be considered as a good substitute for osteoporotic bone when testing orthopaedic implants Although the artificial bone substrate with a density of 0.16 g/cm may be a good substitute for bone, we do recognise that studies conducted with it may need to be validated by further tests with cadaveric bone to produce indisputable results.
A negative test result can also be verified by further testing.
The other associations identified here are not supported by previous analyses and they require further testing with sequences from other slowly evolving nuclear genes.
The change of mitochondrial membrane potential by HMGB1 was further tested with JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide), a more direct indicator of in the mitochondrial membrane potential (ΔΨm) and siRNA against HMGB1 (Fig 3B).
All serum samples that were considered negative by ELISA were further tested with the HCS-GFP neutralization assay as described in Keckler et al. In brief, serum samples were serially diluted from 1 : 40 to 1 : 1280 and then neutralizing antibody (NAb) titers against vaccinia virus were measured using a GFP based assay.
Isolates with mutations in katG detected by the wild-type probe were further tested with another molecular beacon that specifically targeted katG S315T (AGC ACC).
Of these 159 samples, reactivity of 25 exceeded GST background on ELISA, and these were further tested with WB by using SFV-infected or SFV-noninfected tissue culture cell lysates.
The findings can be further tested with grocery retailers.
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