First, when a metagenome contains a very dominant species, this skews the estimates of how much additional material one would expect to find by further sequencing, i.e. the total number of genomes in the metagenome.
If this is the case, it should be borne out by further sequence analysis of viritopes.
By further sequence alignments of the returned mRNA sequences and information from UniGene, we grouped the mRNA transcripts according to their gene products and identified at least 7 distinct egg proteins, somula protein, 53 other hypothetical protein sequences and 10 non-coding mRNAs, all bearing the similar signal motif.
Further, by sequencing of a biofilm identified in a TMV from a hospital water system, we can identify the likely common source of genotypes found in water and in the hospital environment.
16S rDNA sequence-based detection was confirmed in all specimens by further amplification and sequencing of specific rpoB gene fragment (Table 1).
These results could be corroborated by further comparative sequence analysis of genes encoding two phospholipases – plcA and plcB.
The 43 760 predicted protein-coding loci should be examined by further improvement of sequence assembly and gene prediction.
The species names of the fungi identified by the array were further verified by sequencing of the ITS region and the D1/D2 domain of the large-subunit RNA gene; sequences in the two regions were found to be highly specific for a fungal species [ 29, 30].
The representative strains of porcine kobuvirus detected in our study were analyzed further by direct sequencing of their PCR amplicons (216 bp) by using BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).
All the bovine kobuvirus strains detected in our study were analyzed further by direct sequencing of their PCR amplicons with the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) on an automated sequencer (ABI 3100, Applied Biosystems).
