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If this is the case, it should be borne out by further sequence analysis of viritopes.
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However, further sequence analysis by Mutation Surveyor® will increase the accuracy.
On the other hand, normalization of the library by eliminating these highly abundant sequences before further sequence analysis will increase the efficiency of gene discovery, since genes expressed in fewer copies will be more readily detected.
Clone numbers presented in red were used for further sequence analysis.
We used four of the mutated clones for further sequencing analysis.
These results could be corroborated by further comparative sequence analysis of genes encoding two phospholipases – plcA and plcB.
One positive clone, BAC 33-N-3, was investigated further by sequence analysis.
Several strains that were negative by PCR but resistant by MIC testing are being further characterized by sequence analysis of the quinolone resistance-determining region in an attempt to identify other novel mutations that may confer resistance [ 3].
These apparently missing bands may represent an insertion/deletion (indel) in the Glyma04g35130 BURP gene or in BURP gene family members, which is elucidated further by direct sequence analysis (below).
Fourteen different antigens were recognised by cancer patients' sera and were further characterised by sequence analysis, mRNA expression pattern and reactivity with allogeneic sera.
Only abundant operational taxonomic units with unique RFLP and DGGE migration patterns and represented by two or more clones were further characterized by sequence analysis of representative clones.
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