Functional suppression of WWOX by dominant-negatives and small interfering RNA (siRNA) protects cells from apoptosis by tumor necrosis factor, staurosporine, ultraviolet light and ectopic p53 in vitro.
Knockdown or functional suppression of WOX1 by antisense mRNA, small interfering RNA, or dominant negative leads to decreased stability of p53 and apoptotic function [ 26].
These findings are consistent with a functional suppression of survival signalling by miR-142.
Remarkably, nearly all the genes affected by Clo in the enriched muscle component and energy pathways were down-regulated, suggesting functional suppression of energy metabolism and motility.
The BK siRNA#2 was used to test for functional suppression of BK channels.
Our data indicate that functional suppression of Brn-2 together with a region-specific lack of compensation by Brn-1 mediates hypothalamic cell dysfunction by mutant Nhtt.
However, non-mutational functional suppression of BRCA1/2 could result from various mechanisms, such as hypermethylation of the BRCA1 promoter or silencing of BRCA2 by other proteins [ 19, 20].
Viglietta, V., Baecher-Allan, C., Weiner, H. L. & Hafler, D. A. Loss of functional suppression by CD4+CD25+ regulatory T cells in patients with multiple sclerosis.
In contrast, in FV infection, the functional suppression by Tregs mainly targeted CD8+ T cells [ 40], resulting in the development of functional exhausted CD8+ T cells and in high FV loads in lymphatic organs [ 16].
One option would be to additionally assess amount and functional capacities of Tregs by additional markers using flow cytometry and functional suppression assays [4], [13], however this is generally not feasible in large field studies.
