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Lin, H. et al. Discovery of a cytokine and its receptor by functional screening of the extracellular proteome.
The highest score for the discovery of novel genes and degradation pathways has been achieved so far by functional screening of large clone libraries.
Recently, over 130 nitrilases were identified by functional screening of hundreds of environmental DNA libraries, for use in industrial biocatalysis applications [ 9].
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Five positive clones were identified out of nearly 50,000 clones by functional screening, and one of the positive clones was finally selected for further studies due to its high glucose-tolerance (details see below).
A bglMKg gene that encodes a monomeric cold-active glycoside hydrolase family 1 enzyme with an apparent molecular mass of 50 kDa was isolated by the functional screening of a marine metagenomic library.
Widespread known and novel phosphonate utilization pathways in marine bacteria revealed by functional screening and metagenomic analyses.
In this study, we demonstrate the suitability of the vertebrate Danio rerio (zebrafish) for functional screening of novel platelet genes in vivo by reverse genetics.
This result is coherent with the strong selection imposed by the functional screen and is similar to previous results concerning the functional screening of the human gut microbiome [ 13].
Faltejskova, P. et al. Identification and functional screening of microRNAs highly deregulated in colorectal cancer.
In summary, functional screening of the metagenomic library revealed a protease positive clone.
Figure 1 Functional screening of metagenomic library for protease activity on skim milk agar plate.
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