Next, we silenced the gene in Caki-1 and 769-P cells by RNA interference and performed various functional analyses to determine the biological significance of CYP1B1 in RCC progression.
Ingenuity Pathways Analysis (Ingenuity® Systems,, IPA 6.0) Compare Biomarker feature was used to define common molecules for gene expression (Affymetrix U133A, 1095 molecules) and ChIP-chip data (Agilent Technologies, 2135 molecules) followed by functional analyses aimed to determine the similarities between SMAD-module predicted and IPA-generated targets.
Positive hits from putative first/second round screenings have to be further evaluated by functional analyses, for example, in vitro glucose uptake studies similar to the 2-DG assay used here, or in vivo investigations on mice body weight.
The exhaustive analyses of the omics data provides a more rational picture of the actual physiological processes determining the AsA fruit content, and would allow to target sets of co-expressed genes to be further studied by functional analyses.
The most direct way to study gene functions is to create gene knockouts through homologous recombination, followed by functional analyses.
An in-depth exploration of this genome sequence, followed by functional analyses, provided considerable insights into its overall architecture, as well as the functions of particular replicons.
Its enzymatic activity was confirmed by functional analyses.
The data were compared between groups by functional analyses using GenMAPP/MAPPFinder.
Based on the results obtained by functional analyses of CEACAM1 using purified immune cells one would expect an enhanced NK, T and B cell response in Ceacam1−/− mice, favoring the control of MHV-68 infection.
Second, their conclusion was not verified by functional analyses.
