The same cohort was previously investigated by fractionation coupled with surface-enhanced laser desorption/ionisation time-of-flight MS (SELDI-TOF-MS), which resulted in the discovery of differential levels of apolipoprotein C1 and apolipoprotein A1 along with transthyretin and cystatin C [ 8].
The lipophilic fraction (hereafter referred to as LPC) was prepared from a methanol extract of ANE by sequential fractionation with hexane, chloroform and ethyl acetate as described previously by Rayirath et al. [ 83].
Briefly, sRNAs were isolated from the total RNA by size fractionation with 15% Tris-borate-EDTA urea polyacrylamide gel (TBU).
SRNAs with 16-30 nt in length were first separated from the total RNA by size fractionation with 15% TBE urea polyacrylamide gel (TBU).
Briefly, small RNAs with 16-30 nt in length were isolated from the total RNA of WT and MT samples by size fractionation with 15% Tris-borate-EDTA urea polyacrylamide gel (TBU).
The E3 extract was fractioned by continuous liquid/liquid fractionation with hexane, dichloromethane and ethyl acetate for obtaining the F1, F2 and F3 fractions, respectively.
By fractionation of XQLT with chromatographic methods, such as those involving silica gel columns, can further elucidate the mechanisms of XQLT, including the pharmacodynamics and interactions.
In the present study, the extraction was carried out under ultrasonication using 95% ethanol as the solvent, followed by fractionation of same extract with various solvents with increasing polarity and the final extracts were designated as NafE, NafEHx, NafEEa, NafEBu, NafEIn, and NafEW.
It is demonstrated that it is possible to obtain, by fractionation, H. perforatum preparations with significantly increased phloroglucinols-to-naphthodianthrones ratio (up to 95 5).
