Sentence examples for by four changes of from inspiring English sources

They were first placed in 95% ethanol and then in absolute ethanol for 5 min each, followed by four changes of Histosolve for 5 min each.

Next, sample slides were deparaffinised by a 60-min incubation at 60 °C followed by four changes of xylene, three changes of 100% ethanol, two changes of 95% ethanol and storage in tap water.

The proceedings have advanced in fits and starts, however, and have been marked by two changes of judges and a change in venue.

After fixation, the sections were dehydrated in methyl cellosolve (BDH Chemicals) for 24 hours, followed by two changes of 100% ethanol for 24 hours each at 4°C.

The samples were infiltrated gradually (3:1. 1:1, and 1 3 100% ethanol: Historesin, 24 hours each) with Historesin (Leica Canada, Markham, Ontario), followed by two changes of pure Historesin.

Two 10  μm sections were collected and deparaffinised by two changes of xylene and absolute ethanol.

Unbound secondary antibodies were removed by three changes of Tris-buffered saline.

Slides were deparaffinized by three changes of xylene (10 minutes each).

This was followed by two changes of xylene for 30 min each.

Peptides were extracted by three changes of 60%acetonitrile/0.11% TFA, and all extracts were combined and dried in vacuo.

They were dehydrated in steps of 30, 50, 70, 90 and 100% ethanol on ice, followed by two changes of LR-White resin.