Sentence examples for by forty cycles of from inspiring English sources
The cycling conditions were one cycle of denaturation at 95°C for 10 min, followed by forty cycles of amplification (95°C/15 sec, and 60°C/1 min).
PCR reactions were carried out as follows: one cycle of 95°C for 10 min followed by forty cycles of amplification (95°C/15 sec, 65.5°C/30 sec, and 70°C/30 sec).
For the primer design Perfect Probes, the cycle parameters were 95°C for 10 min, followed by forty cycles of 95°C for 10 s and 60 and 72°C for 15 s.
For Roche universal probe library probes the cycle parameters were 95°C for 10 min, followed by forty cycles of 95°C for 15 s and 60°C for 1 min.
The thermal cycling conditions involved an initial denaturation step at 95°C for 4 min followed by forty cycles of 95°C for 10 s, annealing temperature (Table 3) for 10 s, and 72°C for 30 s, followed by a product melting curve to confirm the specificity of amplification.
Two iterative cycles of magnetic bead selections followed by four cycles of fluorescence-activated cell sorting (FACS) were applied.
Nearly 17,500 private homes and 700 buildings containing 20,000 apartments were destroyed by two cycles of war.
About 50 concerts are planned in Mechelen, where Flemish music is complemented by three cycles of 20th-century music by the Paul Collaer Ensemble.
The as-synthesized nanoparticles were isolated by sonication and centrifugation (2,000 g) in dry toluene, followed by three cycles of washing and centrifuging in 2-propanol (IPA).
The kidnapping set off alarms among hundreds of international aid workers who minister to more than 300,000 refugees from Chechnya, where basic food and health services have been largely destroyed by two cycles of war since 1994.
